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Sadly I still get this error

However I did see this before the errors. do you think this is what is causing the audio issues??

Did you place the app into the applications folder?  if you have and it still doesn't show up then I don't know sadly :'( but if you haven't try that! and see if that pops up then! 

Honestly, the folder was already downloaded in the folder.

So after getting into the Library you find the folder "Application Support" first, then you find "Godot"

Here is a better step by step.  

So make sure you are on your desktop, click anywhere on your desktop that is open, then you will see "Go" at the top  on your screen, click on it. Then while holding down “Option”, the Library folder will pop in the list

Okay, and by first post you mean from this thread? sorry wanted to make sure

I feel completely stupid... I feel like I am missing something from all the steps... I started completely over, i deleted the app and followed the steps with the provided link.  I was able to add the application to the microphone permissions but nothing is still working TT_TT

I am trying to figure that out too, I have posted what I have found so far but it looks like we need to use the Terminal and jot down a code to manually give permission to the micrphone.  VeadoTube Mini has done this, so if you know what to replace in the terminal code that may work, the screen shot below is from the VeadoTuber FAQ

I found out how to get to the PNGTuber+ Folder!

Go on your desktop, click on GO on the top of your screen hold down “Option” the Library folder will pop in the list, click to open window, then you just follow the the above folder links. You are going to have a lot of folders so take your time to look to find the name of the folders.

Once you get to the PNGTuberPlus folder you just have to click and drag your images in that folder.

I too am having issues with the Mic, my computer some reason isn't giving permission.  The VeadoTube Mini actually was able to bypass that issue with a terminal but I don't know how to put that in for this application. Below is how you can give permission to the app to use microphone for VeadoTube Mini and it gives the terminal code I just don't know what to replace to get PNGTuber+ to work that way as well.

Library/Application Support/Godot/app_userdata/PNGTuberPlus

So to get to your Library, you go on your desktop, click on GO on the top of your screen hold down “Option” the Library folder will pop in the list, click to open window, then you just follow the the above folder links. You are going to have a lot of folders so take your time to look to find the name of the folders.

Once you get to the PNGTuberPlus folder you just have to click and drag your images in that folder.

The only issue I have right now is my mac is not allowing the App to use my microphone for it, so If someone knows how to give the app permission that be helpful as well (I did check the permissions in security but PNGTuber+ isn't even showing up on the list.)